nasF [2019-06-18 11:57:01]

uroporphyrinogen methyltransferase

Locus: BSU03280

Isoelectric point: 6.52

Molecular weight: 53.70 kDa

Protein length: 483 aa

Gene length: 1452 bp

Function: nitrate respiration

Product: uroporphyrinogen methyltransferase

Essential: no

E.C.: 2.1.1.107

Synonyms: nasBE

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Anaerobic respiration
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of nitrate/ nitrite

Gene

Coordinates: 353,900 355,351

The protein

Catalyzed reaction/ biological activity

• S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1 (according to Swiss-Prot)

Protein family

• precorrin methyltransferase family (according to Swiss-Prot)

Paralogous protein(s)

• YlnD:

Structure

• 1PJQ (CysG from Salmonella enterica, 46% identity to N-terminal domain) PubMed

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • NsrR: repression, PubMed, in NsrR regulon
  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • nasD: expressed under anaerobic conditions (ResD) PubMed

• Genes: nasD-nasE-nasF
  Description: PubMed

  Regulation

  • nasD: expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE03280 (nasF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCATCCTTCCGTCACT, downstream forward: _UP4_TGATGGCAAGCAGCCCTTTT
• BKK03280 (nasF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCATCCTTCCGTCACT, downstream forward: _UP4_TGATGGCAAGCAGCCCTTTT

References

Reviews

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Original publications

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